Main research achievements（Research articles and reviews published in academic journals after 2000）

Relationships of plasma insulin-like peptide 3, testosterone, inhibin, and insulin-like growth factor-I concentrations with scrotal circumference and testicular weight in Japanese Black beef bull calves.
Sakase M, Kitagawa K, Kibushi M, Kawate N, Weerakoon WWPN, Hannan MA, Kohama N, Tamada H.
J Reprod Dev. 2018 Jul 6. doi: 10.1262/jrd.2018-034.

 

Plasma IGF-I, INSL3, testosterone, inhibin concentrations and scrotal circumferences surrounding puberty in Japanese Black beef bulls with normal and abnormal semen.
Weerakoon WWPN, Sakase M, Kawate N, Hannan MA, Kohama N, Tamada H.
Theriogenology. 2018;114:54-62.

 

Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture.
Takahashi M, Honda T, Hatoya S, Inaba T, Kawate N, Tamada H.
J Vet Med Sci. 2018;80(3):532-535.

 

Treatment with the MAPK kinase inhibitor U0126 during the first two hours of in vitro maturation improves bovine oocyte developmental competence.
Shinohara T, Ohta Y, Kawate N, Takahashi M, Sakagami N, Inaba T, Tamada H.
Reprod Domest Anim. 2018;53(1):270-273.

 

Generation of Canine Induced Extraembryonic Endoderm-Like Cell Line That Forms Both Extraembryonic and Embryonic Endoderm Derivatives.
Nishimura T, Unezaki N, Kanegi R, Wijesekera DPH, Hatoya S, Sugiura K, Kawate N, Tamada H, Imai H, Inaba T.
Stem Cells Dev. 2017;26(15):1111-1120.

 

Long-term feeding of hydroalcoholic extract powder of Lepidium meyenii (maca) enhances the steroidogenic ability of Leydig cells to alleviate its decline with ageing in male rats.
Yoshida K, Ohta Y, Kawate N, Takahashi M, Inaba T, Hatoya S, Morii H, Takahashi K, Ito M, Tamada H.
Andrologia. 2018;50(1). doi: 10.1111/and.12803.

 

Feeding hydroalcoholic extract powder of Lepidium meyenii (maca) enhances testicular gene expression of 3β-hydroxysteroid dehydrogenase in rats.
Ohta Y, Kawate N, Inaba T, Morii H, Takahashi K, Tamada H.
Andrologia. 2017;49(10). doi: 10.1111/and.12792.

 

Changes of plasma concentrations of insulin-like peptide 3 and testosterone, and their association with scrotal circumference during pubertal development in male goats.
Hannan MA, Kawate N, Fukami Y, Weerakoon WWPN, Büllesbach EE, Inaba T, Tamada H.
Theriogenology. 2017;92:51-56.

 

Testicular gene expression of steroidogenesis-related factors in prepubertal, postpubertal, and aging dogs.
Ogawa E, Kawate N, Inaba T, Tamada H.
Theriogenology. 2017;90:42-48

 

Effects of long-acting GnRH antagonist, degarelix acetate, on plasma insulin-like peptide 3, testosterone and luteinizing hormone concentrations, and scrotal circumference in male goats.
Hannan MA, Kawate N, Fukami Y, Weerakoon WW, Büllesbach EE, Inaba T, Tamada H.
Theriogenology. 2017;88:228-235.

 

Exposure to Zearalenone During Early Pregnancy Causes Estrogenic Multitoxic Effects in Mice.
Kunishige K, Kawate N, Inaba T, Tamada H.
Reprod Sci. Reprod Sci. 2017;24(3):421-427.

 

Fetal gender prediction based on maternal plasma testosterone and insulin-like peptide 3 concentrations at mid- and late gestation in cattle.

M. Kibushi, N. Kawate, Y. Kaminogo, M.A. Hannan, W.W.P.N. Weerakoon, M. Sakase, M. Fukushima, T. Seyama, T. Inaba, H. Tamada.

Theriogenology. 2016;86(7):1764-73.

 

Acute regulation of plasma insulin-like peptide 3 concentrations by luteinizing hormone in male goats.

Hannan MA, Kawate N, Fukami Y, Pathirana IN, Büllesbach EE, Inaba T, Tamada H.

Theriogenology. 2016;86(3):749-56.

 

Production of feline leukemia inhibitory factor with biological activity in Escherichia coli.

Kanegi R, Hatoya S, Tsujimoto Y, Takenaka S, Nishimura T, Wijewardana V, Sugiura K, Takahashi M, Kawate N, Tamada H, Inaba T.

Theriogenology. 2016;86(2):604-11.

 

Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra.

Tamada H, Adachi N, Kawate N, Inaba T, Hatoya S, Sawada T.

J Vet Med Sci. 2016;78(3):525-8.

 

Expression and localization of epidermal growth factor, transforming growth factor-α and epidermal growth factor receptor in the canine testis.

Tamada H, Takemoto K, Tominaga M, Kawate N, Takahashi M, Hatoya S, Matsuyama S, Inaba T, Sawada T.

J Reprod Dev. 2016 Feb 20;62(1):59-64.

 

Feeding hydroalcoholic extract powder of Lepidium meyenii (maca) increases serum testosterone concentration and enhances steroidogenic ability of Leydig cells in male rats.

Ohta Y, Yoshida K, Kamiya S, Kawate N, Takahashi M, Inaba T, Hatoya S, Morii H, Takahashi K, Ito M, Ogawa H, Tamada H.

Andrologia. 2016;48(3):347-54

 

Plasma insulin-like peptide 3 concentrations are acutely regulated by luteinizing hormone in pubertal Japanese Black beef bulls.

Hannan MA, Fukami Y, Kawate N, Sakase M, Fukushima M, Pathirana IN, Büllesbach EE, Inaba T, Tamada H.

Theriogenology. 2015;84(9):1530-5.

 

Expression analyses of insulin-like peptide 3, RXFP2, LH receptor, and 3β-hydroxysteroid dehydrogenase in testes of normal and cryptorchid dogs.

Hannan MA, Kawate N, Kubo Y, Pathirana IN, Büllesbach EE, Hatoya S, Inaba T, Takahashi M, Tamada H.

Theriogenology. 2015;84(7):1176-84.

 

Improvement of preimplantation development of in vitro-fertilized bovine zygotes by glucose supplementation to a chemically defined medium.

Sakagami N, Nishino O, Adachi S, Umeki H, Uchiyama H, Ichikawa K, Takeshita K, Kaneko E, Akiyama K, Kobayashi S, Tamada H.

J Vet Med Sci. 2014;76(10):1403-5.

 

Clinical study report on milk production in the offspring of a somatic cell cloned Holstein cow.

Takahashi M, Tsuchiya H, Hamano S, Inaba T, Kawate N, Tamada H.

J Reprod Dev. 2013;59(6):595-8.

 

Generation of functional platelets from canine induced pluripotent stem cells.
Nishimura T, Hatoya S, Kanegi R, Sugiura K, Wijewardana V, Kuwamura M, Tanaka M, Yamate J, Izawa

T, Takahashi M, Kawate N, Tamada H, Imai H, Inaba T.

Stem Cells Dev. 2013;22(14):2026-35.

 

Improvement of superovulatory response and pregnancy rate after transfer of embryos recovered from Japanese Black cows fed rumen bypass polyunsaturated fatty acids.

Takahashi M, Sawada K, Kawate N, Inaba T, Tamada H.

J Vet Med Sci. 2013;75(11):1485-90.

 

Insulin-like peptide 3 stimulates testosterone secretion in mouse Leydig cells via cAMP pathway.

Pathirana IN, Kawate N, Büllesbach EE, Takahashi M, Hatoya S, Inaba T, Tamada H.

Regul Pept. 2012;178(1-3):102-6.

 

Delay of ovulation due to diets containing levonorgestrel in cynomolgus monkeys (Macaca fascicularis).

Nakama K, Akune A, Kawate N, Takahashi M, Inaba T, Sameshima H, Tamada H.

J Vet Med Sci. 2012;74(11):1453-60.

 

Factors associated with patency of the uterine cervix in bitches with pyometra.

Tamada H, Kawata N, Kawate N, Inaba T, Kida K, Hatoya S, Akune A, Nakama K, Kohsaka T, Takahashi M, Sawada T.

Res Vet Sci. 2012;93(3):1203-10.

 

Plasma insulin-like peptide 3 and testosterone concentrations in male dogs: changes with age and effects of cryptorchidism.

Pathirana IN, Yamasaki H, Kawate N, Tsuji M, Büllesbach EE, Takahashi M, Hatoya S, Inaba T, Tamada H.

Theriogenology. 2012;77(3):550-7.

 

Normal calves produced after transfer of embryos cultured in a chemically defined medium supplemented with epidermal growth factor and insulin-like growth factor I following ovum pick up and in vitro fertilization in Japanese black cows.

Sakagami N, Umeki H, Nishino O, Uchiyama H, Ichikawa K, Takeshita K, Kaneko E, Akiyama K, Kobayashi S, Tamada H.

J Reprod Dev. 2012;58(1):140-6.

 

Changes in plasma concentrations of insulin-like peptide 3 and testosterone from birth to pubertal age in beef bulls.

Kawate N, Ohnari A, Pathirana IN, Sakase M, Büllesbach EE, Takahashi M, Inaba T, Tamada H.

Theriogenology. 2011;76(9):1632-8.

 

In vitro effects of estradiol-17β, monobutyl phthalate and mono-(2-ethylhexyl) phthalate on the secretion of testosterone and insulin-like peptide 3 by interstitial cells of scrotal and retained testes in dogs.

Pathirana IN, Kawate N, Tsuji M, Takahashi M, Hatoya S, Inaba T, Tamada H.

Theriogenology. 2011;76(7):1227-33.

 

Homozygosity of single nucleotide polymorphisms in the 3′ region of the canine estrogen receptor 1 gene is greater in Toy Poodles than in Miniature Dachshunds and Chihuahuas.

Pathirana IN, Tanaka K, Kawate N, Tsuji M, Hatoya S, Inaba T, Tamada H.

Anim Sci J. 2011;82(3):390-5.

 

Comparison of testosterone and insulin-like peptide 3 secretions in response to human chorionic gonadotropin in cultured interstitial cells from scrotal and retained testes in dogs.

Pathirana IN, Ashida Y, Kawate N, Tanaka K, Tsuji M, Takahashi M, Hatoya S, Inaba T, Tamada H.

Anim Reprod Sci. 2011;124(1-2):138-44.

 

Comparison of plasma concentrations of estradiol-17β and progesterone, and conception in dairy cows with cystic ovarian diseases between Ovsynch and Ovsynch plus CIDR timed AI protocols.

Kawate N, Watanabe K, Uenaka K, Takahashi M, Inaba T, Tamada H.

J Reprod Dev. 2011;57(2):267-72.

 

Induction of fertile oestrus in dioestrous bitches using prostaglandin F2α and a GnRH agonist.

Ohmura M, Torii R, Hatoya S, Sugiura K, Tamada H, Kawate N, Takahashi M, Sawada T, Inaba T.

Vet Rec. 2011;168(25):669.

 

Detection of relaxin mRNA in the corpus luteum, uterus, and uterine cervix in the bitch.

Tamada H, Kawate N, Kawata N, Inaba T, Kida K, Hatoya S, Akune A, Nakama K, Kohsaka T, Sawada T.

J Vet Med Sci. 2010;72(10):1383-6.

 

Analysis of single nucleotide polymorphisms in the 3′ region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas.

Pathirana IN, Tanaka K, Kawate N, Tsuji M, Kida K, Hatoya S, Inaba T, Tamada H.

J Reprod Dev. 2010;56(4):405-10.

 

Epidermal growth factor, transforming growth factor-alpha, and epidermal growth factor receptor expression and localization in the canine endometrium during the estrous cycle and in bitches with pyometra.

Kida K, Maezono Y, Kawate N, Inaba T, Hatoya S, Tamada H.

Theriogenology. 2010;73(1):36-47.

 

Canine oocyte maturation in culture: significance of estrogen and EGF receptor gene expression in cumulus cells.

Hatoya S, Sugiyama Y, Nishida H, Okuno T, Torii R, Sugiura K, Kida K, Kawate N, Tamada H, Inaba T.

Theriogenology. 2009;71(4):560-7.

 

Ovsynch plus CIDR protocol for timed embryo transfer in suckled postpartum Japanese Black beef cows.

Kawate N, Sakase M, Watanabe K, Fukushima M, Noda M, Takeda K, Ueno S, Inaba T, Kida K, Tamada H, Sawada T.

J Reprod Dev. 2007;53(4):811-7.

 

Lactoferrin expression in the canine uterus during the estrous cycle and with pyometra.

Kida K, Baba E, Torii R, Kawate N, Hatoya S, Wijewardana V, Sugiura K, Sawada T, Tamada H, Inaba T.

Theriogenology. 2006;66(5):1325-33.

 

Inhibitory effects of CIDR-based ovulation-synchronization protocols on uterine PGF2alpha secretion at the following luteal phase in early postpartum non-cycling beef cows.

Sakase M, Kawate N, Nakagawa C, Fukushima M, Noda M, Takeda K, Ueno S, Inaba T, Kida K, Tamada H, Sawada T.

J Reprod Dev. 2006;52(4):497-502.

 

Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocytes.

Hatoya S, Sugiyama Y, Torii R, Wijewardana V, Kumagai D, Sugiura K, Kida K, Kawate N, Tamada H, Sawada T, Inaba T.

Theriogenology. 2006;66(5):1083-90.

 

Preventive effects of CIDR-based protocols on premature ovulation before timed-AI in Ovsynch in cycling beef cows.

Sakase M, Kawate N, Nakagawa C, Fukushima M, Noda M, Takeda K, Ueno S, Inaba T, Kida K, Tamada H, Sawada T.

Vet J. 2007;173(3):691-3.

 

Reduction of mucin-1 gene expression associated with increased Escherichia coli adherence in the canine uterus in the early stage of dioestrus.

Ishiguro K, Baba E, Torii R, Tamada H, Kawate N, Hatoya S, Wijewardana V, Kumagai D, Sugiura K, Sawada T, Inaba T.

Vet J. 2007;173(2):325-32.

 

Relations between plasma IGF-I concentrations during treatment with CIDR-based or Ovsynch protocol for timed AI and conception in early postpartum Japanese black beef cows.

Kawate N, Sakase M, Seo Y, Fukushima M, Noda M, Takeda K, Ueno S, Inaba T, Kida K, Tamada H, Sawada T.

J Reprod Dev. 2006;52(1):81-9.

 

Isolation and characterization of embryonic stem-like cells from canine blastocysts.

Hatoya S, Torii R, Kondo Y, Okuno T, Kobayashi K, Wijewardana V, Kawate N, Tamada H, Sawada T, Kumagai D, Sugiura K, Inaba T.

Mol Reprod Dev. 2006;73(3):298-305.

 

Effect of CIDR-based protocols for timed-AI on the conception rate and ovarian functions of Japanese Black beef cows in the early postpartum period.

Sakase M, Seo Y, Fukushima M, Noda M, Takeda K, Ueno S, Inaba T, Tamada H, Sawada T, Kawate N.

Theriogenology. 2005;64(5):1197-211.

 

Induction of fertile oestrus in bitches by an intranasal spray of gonadotrophin-releasing hormone agonist.

Hatoya S, Torii R, Wijewardana V, Kumagai D, Sugiura K, Kawate N, Tamada H, Sawada T, Inaba T.

Vet Rec. 2006;158(11):378-9.

 

Detection of transforming growth factor-alpha and epidermal growth factor receptor mRNA and immunohistochemical localization of the corresponding proteins in the canine uterus during the estrous cycle.

Tamada H, Tominaga M, Kida K, Kawate N, Inaba T, Matsuyama S, Sawada T.

Histol Histopathol. 2005;20(3):817-24.

 

Adenomyosis with severe inflammation in the uterine cervix in a dog.

Tamada H, Kawate N, Inaba T, Kuwamura M, Maeda M, Kajikawa T, Sawada T.

Can Vet J. 2005 Apr;46(4):333-4.

 

The effects of the aromatase inhibitor fadrozole hydrochloride on fetuses and uteri in late pregnant rats.

Tamada H, Shimizu Y, Inaba T, Kawate N, Sawada T.

J Endocrinol. 2004;180(2):337-45.

 

Studies on the regulation of expression of luteinizing hormone receptor in the ovary and the mechanism of follicular cyst formation in ruminants.

Kawate N.

J Reprod Dev. 2004;50(1):1-8.

 

Enhancement of prostacyclin synthesis at the beginning of formation of caprine corpora lutea.

Kawate N, Tsuji M, Tamada H, Inaba T, Sawada T.

Mol Reprod Dev. 2004;67(3):308-12.

 

Improved conception in timed-artificial insemination using a progesterone-releasing intravaginal device and Ovsynch protocol in postpartum suckled Japanese Black beef cows.

Kawate N, Itami T, Choushi T, Saitoh T, Wada T, Matsuoka K, Uenaka K, Tanaka N, Yamanaka A, Sakase M, Tamada H, Inaba T, Sawada T.

Theriogenology. 2004;61(2-3):399-406.

 

Effect of ovarian hormones on periodical changes in immune resistance associated with estrous cycle in the beagle bitch.

Sugiura K, Nishikawa M, Ishiguro K, Tajima T, Inaba M, Torii R, Hatoya S, Wijewardana V, Kumagai D, Tamada H, Sawada T, Ikehara S, Inaba T.

Immunobiology. 2004;209(8):619-27.

 

Delayed implantation induced by fadrozole hydrochloride in rats.

Tamada H, Mizuta Y, Kawate N, Inaba T, Sawada T.

Contraception. 2003;68(1):65-8.

 

Expression of estrogen receptor alpha and beta genes in the mediobasal hypothalamus, pituitary and ovary during the canine estrous cycle.

Hatoya S, Torii R, Kumagai D, Sugiura K, Kawate N, Tamada H, Sawada T, Inaba T.

Neurosci Lett. 2003;347(2):131-5.

 

Detection of transforming growth factor-alpha and epidermal growth factor receptor mRNA and immunohistochemical localization of their proteins in the ovine uterus during the early implantation period.

Tamada H, Tsubutani D, Kawate N, Inaba T, Matsuyama S, Imakawa K, Sakai S, Christenson RK, Sawada T.

Histochem J. 2002;34(8-9):383-90.

 

Long-term prevention of estrus in the bitch and queen using chlormadinone acetate.

Tamada H, Kawate N, Inaba T, Sawada T.

Can Vet J. 2003;44(5):416-7.

 

Changes of messenger RNAs encoding vascular endothelial growth factor and its receptors during the development and maintenance of caprine corpora lutea.

Kawate N, Tsuji M, Tamada H, Inaba T, Sawada T.

Mol Reprod Dev. 2003;64(2):166-71.

 

Enhancement of aromatase gene expression in the mediobasal hypothalamus during anestrus in the beagle bitch.

Inaba T, Namura T, Tani H, Matsuyama S, Torii R, Kawate N, Tamada H, Hatoya S, Kumagai D, Sugiura K, Sawada T.

Neurosci Lett. 2002;333(2):107-10.

 

Inhibition of luteinizing hormone receptor expression during the development of caprine corpora lutea by administration of gonadotropin-releasing hormone antagonist.

Kawate N, Tsuji M, Tamada H, Inaba T, Sawada T.

Mol Reprod Dev. 2002;63(4):444-50.

 

Enhancement of aromatase gene expression in the mediobasal hypothalamus during anestrus in the beagle bitch.

Inaba T, Namura T, Tani H, Matsuyama S, Torii R, Kawate N, Tamada H, Hatoya S, Kumagai D, Sugiura K, Sawada T.

Neurosci Lett. 2002;333(2):107-10.

 

Change in concentrations of luteinizing hormone subunit messenger ribonucleic acids in the estrous cycle of beef cattle.

Kawate N, Akiyama M, Suga T, Inaba T, Tamada H, Sawada T, Mori J.

Anim Reprod Sci. 2001;68(1-2):13-21.

 

The effect of transforming growth factor-alpha on the progression of decidualization in rats.

Tamada H, Sakaguchi H, Inaba T, Kawate N, Sawada T.

Life Sci. 2001;69(13):1549-58.

 

Mechanism of ovarian cyst formation in ruminants.

Noritoshi Kawate

J Jpn Vet Med Assoc. 2001;54(2):79-84. (In Japanese)

 

Roles of pulsatile release of LH in the development and maintenance of corpus luteum function in the goat.

Kawate N, Monrita N, Tsuji M, Tamada H, Inaba T, Sawada T.

Theriogenology. 2000;54(7):1133-43.

 

Anovulation to a luteinising hormone surge in an aged goat with follicular cysts.

Kawate N, Morita N, Tsuji M, Tamada H, Inaba T, Sawada T.

Vet Rec. 2000;147(10):272-4.

 

Epidermal growth factor (EGF) in the goat uterus: immunohistochemical localization of EGF and EGF receptor and effect of EGF on uterine activity in vivo.

Tamada H, Yoh C, Inaba T, Takano H, Kawate N, Sawada T.

Theriogenology. 2000;54(1):159-69.

 

Seasonal changes in the hypothalamo-pituitary-testes axis of the Japanese wood mouse (Apodemus speciosus).

Kuwahara S, Mizukami T, Omura M, Hagihara M, Iinuma Y, Shimizu Y, Tamada H, Tsukamoto Y, Nishida T, Sasaki F.

Anat Rec. 2000;260(4):366-72.

 

Tumor necrosis factor-alpha and its receptor in bovine corpus luteum throughout the estrous cycle.

Sakumoto R, Berisha B, Kawate N, Schams D, Okuda K.

Biol Reprod. 2000;62(1):192-9.