• Adherence of Clostridium perfringens spores to human intestinal epithelial Caco-2 cells. Sakanoue H, Nakano T, Sano K, Yasugi M, Monma C, Miyake M. FEMS Microbiol. Lett. 365, in press, 2018 (doi: 10.1093/femsle/fny016).
  • An enhanced green fluorescence protein (EGFP)-based reporter assay for quantitative detection of sporulation in Clostridium perfringens SM101. Wakabayashi Y, Nariya H, Yasugi M, Kuwahara T, Sarker MR, Miyake M. Int. J. Food Microbiol. 291: 144-150, 2018.
  • Evaluation of multinomial logistic regression models for predicting causative pathogens of food poisoning cases. Inoue H, Suzuki T, Hyodo M, Miyake M. J. Vet. Med. Sci. 80: 1223-1227, 2018.
  • Effect of sublethal heat treatment on the later stage of germination-to-outgrowth of Clostridium perfringens spores. Sakanoue H, Yasugi M, Miyake M. Microbiol Immunol. 62: 418-424, 2018.




  • The transcriptional profile during deoxycholate-induced sporulation in a Clostridium 
perfringens isolate from foodborne illness. 
Yasugi M, Okuzaki D, Kuwana R, Takamatsu H, Fujita M, Sarker MR, Miyake M. Appl. Environ. Microbiol. 82: 2929-2942, 2016.




  • Voltammetric detection and profiling of isoprenoid quinones hydrophobically transferred from bacterial cells. Le DQ, Morishita A, Tokonami S, Nishino T, Shiigi H, Miyake M, Nagaoka T. Anal. Chem. 87: n8416-n8423, 2015.
  • In vitro cytotoxicity induced by Clostridium perfringens isolate carrying a chromosomal cpe gene is exclusively dependent on sporulation and enterotoxin production. Yasugi M, Sugahara Y, Hoshi H, Kondo K, Talukdar PK, Sarker MR, Yamamoto S, Kamata Y, Miyake MMicrob. Pathog. 85: 1-10, 2015.
  • Influenza A Virus-Induced Expression of a GalNAc Transferase, GALNT3, via MicroRNAs Is Required for Enhanced Viral Replication. Nakamura S, Horie M, Daidoji T, Honda T, Yasugi M, Kuno A, Komori T, Okuzaki D, Narimatsu H, Nakaya T, Tomonaga K. J. Virol. 90: 1788-1801, 2015.
  • Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance between Hemagglutinin Acid Stability and Endosomal pH. Daidoji T, Watanabe Y, Ibrahim MS, Yasugi M, Maruyama H, Masuda T, Arai F, Ohba T, Honda A, Ikuta K, Nakaya T. J. Biol. Chem. 290: 10627-10642, 2015.




  • A human monoclonal antibody derived from a vaccinated volunteer recognizes heterosubtypically a novel epitope on the hemagglutinin globular head of H1 and H9 influenza A viruses. Boonsathorn N, Panthong S, Koksunan S, Chittaganpitch M, Phuygun S, Waicharoen S, Prachasupap A, Sasaki T, Kubota-Koketsu R, Yasugi M, Ono K, Arai Y, Kurosu T, Sawanpanyalert P, Ikuta K, Watanabe Y. Biochem. Biophys. Res. Commun. 452: 865-870, 2014.
  •  Human monoclonal antibodies derived from a patient infected with 2009 pandemic influenza A virus broadly cross-neutralize group 1 influenza viruses. Pan Y, Sasaki T, Kubota-Koketsu R, Inoue Y, Yasugi M, Yamashita A, Ramadhany R, Arai Y, Du A, Boonsathorn N, Ibrahim MS, Daidoji T, Nakaya T, Ono K, Okuno Y, Ikuta K, Watanabe Y. Biochem. Biophys. Res. Commun. 450: 42-48, 2014.
  • Autophagy activation by interferon-γ via the p38 mitogen-activated protein kinase signalling pathway is involved in macrophage bactericidal activity. Matsuzawa T, Fujiwara E, Washi Y. Immunology. 141(1):61-69, 2014.




  •  Emerging antigenic variants at the antigenic site Sb in pandemic A(H1N1)2009 influenza virus in Japan detected by a human monoclonal antibody. Yasugi M, Kubota-Koketsu R, Yamashita A, Kawashita N, Du A, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno Y, Nakaya T, Ikuta K. PLoS One. 8: e77892, 2013.
  • Human monoclonal antibodies broadly neutralizing against influenza B virus. Yasugi M, Kubota-Koketsu R, Yamashita A, Kawashita N, Du A, Sasaki T, Nishimura M, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno Y, Nakaya T, Ikuta K. PLoS Pathog. 9: e1003150, 2013.
  •  Observation of autophagosome maturation in the interferon-γ-primed and lipopolysaccharide-activated macrophages using a tandem fluorescent tagged LC3. Fujiwara E, Washi Y, Matsuzawa TJ. Immunol. Methods. 394: 100-106, 2013.




  • Tropism of Pandemic 2009 H1N1 Influenza a Virus. Ramadhany R, Yasugi M, Nakamura S, Daidoji T, Watanabe Y, Takahashi K, Ikuta K, Nakaya T. Front. Microbiol. 3:128, 2012.
  • Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in Japan between 2009 and 2010. Yasugi M, Nakamura S, Daidoji T, Kawashita N, Ramadhany R, Yang CS, Yasunaga T, Iida T, Horii T, Ikuta K, Takahashi K, Nakaya T. PLoS One. 7: e30946, 2012.
  • IFN-gamma elicits macrophage autophagy via the p38 MAPK signaling pathway. Matsuzawa T, Kim BH, Shenoy AR, Kamitani S, Miyake M, Macmicking JD. J Immunol. 2012. 189: 813-818, 2012.




  • Association of Bordetella dermonecrotic toxin with the extracellular matrix. Fukui-Miyazaki A, Kamitani S, Miyake M, and Horiguchi Y. BMC Microbiol. 2010. 10: 247, 2010.

  • Characterization of the membrane-targeting domain in Pasteurella multocida toxin. Kamitani S, Kitadokoro K, Miyazawa M, Toshima H, Fukui A, Abe H, Miyake M, and Horiguchi Y. J. Biol. Chem. 2010. 285: 25467-75, 2010.

  • Clostridium perfringens enterotoxin interacts with claudins by electrostatic attraction.
     Kimura J, Abe H, Kamitani S, Toshima H, Fukui A, Miyake M, Kamata Y, Sugita-Konishi Y, Yamamoto S, and Horiguchi Y. J. Biol. Chem. 285: 401-408, 2010.

  • Highly pathogenic H5N1 avian influenza virus induces extracellular Ca2+ influx, leading to apoptosis in avian cells. Ueda (Yasugi) M, Daidoji T, Du A, Yang CS, Ibrahim MS, Ikuta K, Nakaya T. J. Virol. 84: 3068-78, 2010.




  • Targeting of the GTPase Irgm1 to the phagosomal membrane via PtdIns(3,4)P2 and PtdIns(3,4,5)P3 promotes immunity to mycobacteria. Tiwari S, Choi H-P, Matsuzawa T, Pypaert M, and MacMicking JD. Nat. Immunol. 10: 907-917, 2009. 
  • Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput sequencing approach. Nakamura S, Yang CS, Sakon N, Ueda (Yasugi) M, Tougan T, Yamashita A, Goto N, Takahashi K, Yasunaga T, Ikuta K, Mizutani T, Okamoto Y, Tagami M, Morita R, Maeda N, Kawai J, Hayashizaki Y, Nagai Y, Horii T, Iida T, Nakaya T. PLoS One. 4: e4219, 2009.

  • Detection of circulating Asian H5N1 viruses by a newly established monoclonal antibody. Du A, Daidoji T, Koma T, Ibrahim MS, Nakamura S, de Silva UC, Ueda (Yasugi) M, Yang CS, Yasunaga T, Ikuta K, Nakaya T. Biochem Biophys Res Commun. 378: 197-202, 2009.




  • Molecular cloning of full-length Dmrt1 cDNA of reeves turtle (Chinemys reevesii). Hoshi H and Nakao A. J. Vet. Med. Sci. 70: 687-692, 2008.

  • Effect of Gb3 in lipid rafts in resistance to Shiga-like toxin of mutant Vero cells. Hanashima T, Miyake M, Yahiro K, Iwamaru Y, Ando A, Morinaga N, Noda M. Microb Pathog. 45: 124-133, 2008.

  • Vitellogenin, a biomarker for environmental estrogenic pollution, of Reeves’ pond turtle: Analysis of similarity for its amino acid sequence and cognate mRNA expression after exposure to estrogen. Tada N, Nakao A, Hoshi H, Saka M and Kamata Y. J. Vet. Med. Sci. 70: 227-234, 2008.

  • The morphological changes in cultured cells caused by Bordetella pertussis adenylate cyclase toxin. Ohnishi H, Miyake M, Kamitani S, and Horiguchi Y. FEMS Microbiol. Lett. 279: 174-179, 2008.

  • A colorimetric assay for studying effector secretion through the bacterial type III secretion system. Miyake M, Sakane S, Kobayashi C, Hanajima-Ozawa M, Fukui A, Kamitani S, and Horiguchi Y. FEMS Microbiol. Lett. 278: 36-42, 2008.

  • H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells. Daidoji T, Koma T, Du A, Yang CS, Ueda (Yasugi) M, Ikuta K, Nakaya T. J. Virol. 82: 11294-11307, 2008.

  • Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus. Ueda (Yasugi) M, Yamate M, Du A, Daidoji T, Okuno Y, Ikuta K, Nakaya T. Virus Res. 136: 91-97, 2008.




  • Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes recruit a junctional protein, zonula occludens-1, to actin tails and pedestals. Hanajima-Ozawa M, Matsuzawa T, Fukui A, Kamitani S, Ohnishi H, Abe A, Horiguchi Y, and Miyake MInfect. Immun. 75: 565-573, 2007.
  • Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin. Kitadokoro K, Kamitani S, Miyazawa M, Hanajima-Ozawa M, Fukui A, Miyake M, and Horiguchi Y. Proc. Natl. Acad. Sci. USA. 104: 5139-5144, 2007.

  • Emerging themes in IFN-gamma-induced macrophage immunity by the p47 and p65 GTPase families. Shenoy AR, Kim BH, Choi H-P, Matsuzawa T, Tiwari S, and MacMicking JD. Immunobiology 212: 771-784, 2007.

  • Characterization of Dog Allegens, Can f1 and Can f2: II. Comparison of Can f1 with Can f2 regarding their biochemical and immunological properties. Kamata Y, Miyanomae A, Nakayama E, Miyanomae T, Tajima T, Nishimura K, Tada T, and Hoshi HInt. Arch. Allergy Immunol. 142: 301-308, 2007.

  • Characterization of dog allergens, Can f1 and Can f2: I. Preparation of their recombinant proteins and antibodies. Kamata Y, Miyanomae A, Nakayama E, Miyanomae T, Tajima T, and Hoshi H. Int. Arch. Allergy Immunol. 142: 291-300, 2007. 




  • Assembly of the type III secretion apparatus of enteropathogenic Escherichia coli. Ogino T, Ohno T, Sekiya K, Kuwae A, Matsuzawa T, Nonaka T, Fukuda H, Imajoh-Ohmi S, and Abe AJ. Bacteriol188: 2801-2811, 2006.
  • BopC is a novel type III effector secreted by Bordetella bronchiseptica and has a critical role in type III-dependent necrotic cell death. Kuwae A, Matsuzawa T, Ishikawa N, Abe H, Nonaka T, Fukuda H, Imajoh-Ohmi S, and Abe A. J. Biol. Chem. 281: 6589-6600, 2006.




  • Binding of intimin with Tir on the bacterial surface is prerequisite for the barrier disruption induced by enteropathogenic Escherichia coliMiyake M, Hanajima M, Matsuzawa T, Kobayashi C, Minami M, Abe A, and Horiguchi Y. Biochem. Biophys. Res. Commun. 337: 922-927, 2005. 
  • Enteropathogenic Escherichia coli type III effectors EspG and EspG2 alter epithelial paracellular permeability. Matsuzawa T, Kuwae A, and Abe A. Infect. Immun. 73: 6283-6289, 2005.

  • Type-III effectors: sophisticated bacterial virulence factors. Abe A, Matsuzawa T, and Kuwae A. Comptes Rendus Biologies 328: 413-428, 2005.

  • Production of monoclonal antibodies against Chinemys reevesii turtle vitellogenin and their usage for comparison of biochemical and immunological characters of vitellogenins and yolk proteins of freshwater turtles. Kamata Y, Tada N, Saka M, Minakawa F, and Hoshi HComp. Biochem. Physiol. B Biochem. Mol. Biol. 142: 233-238, 2005.  




  • Bordetella dermonecrotic toxin undergoes proteolytic processing to be translocated from a dynamin-related endosome into the cytoplasm in an acidification-independent manner. Matsuzawa T, Fukui A, Kashimoto T, Nagao K, Oka K, Miyake M, and Horiguchi Y. J. Biol. Chem. 279: 2866-2872.
  • The type III secreted protein BopD in Bordetella bronchiseptica is complexed with BopB for pore formation on the host plasma membrane. Nogawa H, Kuwae A, Matsuzawa T, and Abe A. J. Bacteriol. 186: 3806-3813, 2004.

  • Enteropathogenic Escherichia coli activates the RhoA signaling pathway via the stimulation of GEF-H1. Matsuzawa T, Kuwae A, Yoshida S, Sasakawa C, and Abe A. EMBO J. 23: 3570-3582, 2004.

  • Comparative analyses of serum vitellogenin levels in male and female Reeves’ pond turtles (Chinemys reevesii) by an immunological assay. Tada N, Saka M, Ueda Y, Hoshi H, Uemura T, and Kamata Y. J. Comp. Physiol. [B]. 174: 13-20, 2004.




  • Association of absence of intestinal oxalate degrading bacteria with urinary calcium oxalate stone formation. Mikami K, Akakura K, Takei K, Ueda T, Mizoguchi M, Noda M, Miyake M, and Ito H. Int. J. Urol. 10: 293-296.
  •  Effects of 17beta-estradiol, bisphenol A and tributyltin chloride on germ cells of Caenorhabditis elegansHoshi H, Kamata Y, and Uemura T. J. Vet. Med. Sci. 65: 881-885, 2003.




  • Inhibition by apple polyphenols of ADP-ribosyltransferase activity of cholera toxin and toxin-induced fluid accumulation in mice. Saito T, Miyake M, Toba M, Okamatsu H, Shimizu S, and Noda M. Microbiol. Immunol. 46: 249-255, 2002.
  • Identification in traditional herbal medications and confirmation by synthesis of factors that inhibit cholera toxin-induced fluid accumulation. Oi H, Matsuura D, Miyake M, Ueno M, Takai I, Yamamoto T, Kubo M, Moss J, and Noda M. Proc. Natl. Acad. Sci. USA. 99: 3042-3046, 2002.

  • Clinical and pathologic characteristics of nontyphoidal salmonella encephalopathy. Arii J, Tanabe Y, Miyake M, Mukai T, Matsuzaki M, Niinomi N, Watanabe H, Yokota Y, Kohno Y, and Noda M. Neurology 58: 1641-1645, 2002.

  • Identification of a receptor-binding domain of Bordetella dermonecrotic toxin. Matsuzawa T,  Kashimoto T, Katahira J, and Horiguchi Y. Infect.  Immun. 70: 3427-3432, 2002.

  •  In vivo modifications of small GTPase Rac and Cdc42 by Bordetella dermonecrotic toxin. Masuda M, Minami M, Shime H, Matsuzawa T, and Horiguchi Y. Infect. Immun. 70: 998-1001, 2002.

  • Characterization of a neutralizing monoclonal antibody against botulinum ADP-ribosyltransferase, C3 exoenzyme. Kamata Y, Hoshi H, Choki H, and Kozaki S. J. Vet. Med. Sci. 64: 767-771, 2002.

  • An outbreak of gastroenteritis in Osaka, Japan due to Escherichia coli serogroup O166:H15 that had a coding gene for enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1). Zhou Z, Ogasawara J, Nishikawa Y, Seto Y, Helander A, Hase A, Irutani N, Nakamura H, Arikawa K, Kai A, Kamata Y, Hoshi H, and Haruki K. Epidemiol. Infect128: 363-371, 2002.




  • An inhibitory factor for cell-free protein synthesis from Salmonella enteritidis exhibits cytopathic activity against Chinese hamster ovary cells. Iwamaru Y, Miyake M, Arii J, Tanabe Y, and Noda M. Microb. Pathog. 31: 283-293, 2001.
  • Acute encephalopathy associated with nontyphoidal salmonellosis. Arii J, Tanabe Y, Miyake M, Noda M, Takahashi Y, Hishiki H, and Kohno Y. J. Child Neurol. 16: 539-540, 2001.




  • Novel proteinaceous toxins from the box jellyfish (sea wasp) Carybdea rastoniNagai H, Takuwa K, Nakao M, Ito E, Miyake M, Noda M, and Nakajima T. Biochem. Biophys. Res. Commun. 275: 582-588, 2000.
  •  Binding of avian ovomucoid to shiga-like toxin type 1 and its utilization for receptor analog affinity chromatography. Miyake M, Utsuno E, and Noda M. Anal. Biochem. 281: 202-208, 2000.
  • Haemophilus influenzae infection and Guillain-Barré syndrome. Mori M, Kuwabara S, Miyake M, Noda M, Kuroki H, Kanno H, Ogawara K, and Hattori T. Brain 123: 2171-2178, 2000.

  •  Enhancement of poly-adenosine diphosphate-ribosylation in human hepatocellular carcinoma. Nomura F, Yaguchi M, Togawa A, Miyazaki M, Isobe K, Miyake M, Noda M, and Nakai T. J. Gastroenterol. Hepatol. 15: 529-535, 2000.

  • Activation of rho through a cross-link with polyamines catalyzed by Bordetella dermonecrotizing toxin. Masuda M, Betancourt L, Matsuzawa T, Kashimoto T, Takao T, Shimonishi Y, and Horiguchi Y. EMBO J. 19: 521-530, 2000.

  • Cloning of the gene coding for Staphylococcus hyicus exfoliative toxin B and its expression in Escherichia coli. Watanabe T, Sato H, Hatakeyama Y, Matsuzawa T, Kawai M, Aizawa C, Danbara H, and Maehara N. J. Bacteriol. 182: 4101-4103, 2000.