Tsukamoto, a 2nd year graduate student in the doctoral program, was appointed as a research fellowship for young scientists, DC1, of the Japan Society for the Promotion of Science in April 2019.

※Awarded to excellent young researchers, these fellowships offer the fellows an opportunity to focus on a freely chosen research topic based on their own innovative ideas. Ultimately, the program works to foster and secure excellent researchers.（From the website of Japan Society for the Promotion of Science）

Mr. Alam, a foreign student who was a member of the PhD program until March 2019, accepted his paper in The Journal of Veterinary Medical Science.

In this study, we demonstrated that a combination of human embryo medium and bovine fetal serum (FBS) can effectively produce feline blastocyst stage embryos by culturing feline fertilized oocyte. The use of commercially available media with quality control enabled stable cultivation of feline embryos.

Feline embryo development in commercially available human media supplemented with fetal bovine serum.
Alam ME, Iwata J, Fujiki K, Tsujimoto Y, Kanegi R, Kawate N, Tamada H, Inaba T, Sugiura K, Hatoya S.
J Vet Med Sci. 2019 Feb 19. doi: 10.1292/jvms.18-0335.

Tsujimoto’s thesis was accepted into the Journal of Reproduction and Development.
In recent years, in vitro fertilization in cats has been studied for use in endangered cats. In our laboratory, we have succeeded in obtaining blastocyst stage embryos by in vitro maturation (IVM) of cat oocytes and microinsemination (ICSI) using mature sperm. In this study, ICSI with immature sperm was successfully performed to produce embryos with the aim of improving in vitro fertilization methods in cats, and effective utilization of cat gene resources is expected.

Development of feline embryos produced by Piezo-actuated intracytoplasmic sperm injection of elongated spermatids.
Tsujimoto Y, Fujiki K, Alam ME, Tsukamoto M, Azuma R, Kanegi R, Anzai M, Inaba T, Sugiura K, Hatoya S.
J Reprod Dev. 2019 Feb 18. doi: 10.1262/jrd.2018-119.

Graduate student Tsukamoto’s reserach was published in Stem Cells and Development.

The researchers generated canine iPS cells using Sendai virus vectors that are automatically removed. This enables the production of virus-free canine iPS cells. It is expected that research for clinical application will be promoted in the future.

Generation of Footprint-Free Canine Induced Pluripotent Stem Cells Using Auto-Erasable Sendai Virus Vector.
Tsukamoto M, Nishimura T, Yodoe K, Kanegi R, Tsujimoto Y, Alam ME, Kuramochi M, Kuwamura M, Ohtaka M, Nishimura K, Nakanishi M, Inaba T, Sugiura K, Hatoya S.
Stem Cells Dev. 2018 Nov 15;27(22):1577-1586. doi: 10.1089/scd.2018.0084. Epub 2018 Oct 11.

Canine iPS cells                Undifferentiated marker

Associate Professor Hatoya and graduate student Tsukamoto presented their findings at the Tissue Engineering and Regenerative Medicine International Society (TERMIS) meeting held in Kyoto on September 4-7, 2018.

Hatoya made an oral presentation titled “Generation of canine and feline pluripotent stem cells for application in regulatory medicine”

Tsukamoto presented a poster titled “Induction of genes free canine induced pluripotent stem cells by using sendai virus vector”.

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